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Contributor Information

  • Name Sandra Van Schaeybroeck
  • Institute Queen's University Belfast

Tool Details

  • Tool name: HCT 116-I6 Invasive Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Cancer type: Colorectal cancer
  • Disease: Cancer
  • Growth properties: Invasion, migration
  • Model: Cancer Model
  • Conditional: No
  • Research area: Cancer ; Cell Structure and Motility ; Drug Discovery & Development
  • Production details: HCT 116 cells were incubated in a Matrigel Invasion Chambers (MIC) for 72-96h. The cells that invaded through to the bottom chamber were collected and designated as ‘Invasive 1’ (I1). These cells were propagated and repeatedly passed through the MIC until highly invasive HCT116-I6 cells were selected.
  • Cellosaurus ID: CVCL_HG06

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.
  •   EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.
  •   Dunne et al. 2014. Clin Cancer Res. 20(1):164-75. PMID: 24170546.
  •   AXL is a key regulator of inherent and chemotherapy-induced invasion and predicts a poor clinical outcome in early-stage colon cancer.