CELL LINES
Contributor Information
- Name Sandra Van Schaeybroeck
- Institute Queen's University Belfast
Tool Details
- Tool name: HCT 116 ADAM17 Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HCT 116
- Organism: Human
- Tissue: Colon
- Cancer type: Digestive / Gastrointestinal cancer
- Disease: Cancer
- Growth properties: Invasion, migration
- Model: Knock-In
- Conditional: No
- Description: Chemotherapy (5-fluorouracil) treatment has been shown to result in acute increases in transforming growth factor-a, amphiregulin, and heregulin ligand shedding in vitro and in vivo correlating with significantly increased ADAM-17 activity. Small interfering RNAmediated silencing and pharmacologic inhibition confirmed that ADAM-17 was the principal ADAM involved in this prosurvival response. HCT 116 ADAM17 Cell Line showed that overexpression of ADAM-17 significantly decreases the effect of chemotherapy on tumor growth and apoptosis.
- Research area: Cancer; Drug development
- Production details: HCT116 cells were cotransfected with 10 ÄÂÄ?g of a plasmid containing hemagglutinin (HA)-tagged full-length mouse ADAM-17 (HA-ADAM-17) and a construct expressing a puromycin resistance gene. Stably transfected cells were selected and maintained in medium supplemented with 1 ÄÂÄ?g/mL puromycin.
- Cellosaurus ID: CVCL_HG03
- Additional notes: Offered under licence from the Spanish National Research Council (CSIC)
- For Research Use Only
Target Details
- Target: mouse ADAM17 (TACE)
Application Details
Handling
- Format: Frozen
- Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Kyula et al. 2010. Clin Cancer Res. 16(13):3378-89. PMID: 20570921.
- • Chemotherapy-induced activation of ADAM-17: a novel mechanism of drug resistance in colorectal cancer.
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