CELL LINES
Contributor Information
- Name Gillian Tozer
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Mouse fibrosarcoma VEGF164 Cell Line
- Alternate names: VEGF-A, VEGF12, VEGF164, VEGF188
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MEF
- Organism: Mouse
- Tissue: Embryo
- Disease: Cancer
- Growth properties: Adherent
- Model: Immortalised Line
- Conditional: No
- Description: Mouse fibrosarcoma cell lines that are capable of expressing all vascular endothelial growth factor (VEGF) isoforms (control) or only single isoforms of VEGF (VEGF120, VEGF164, or VEGF188) were developed under endogenous VEGF promoter control. Using Cre/Lox technology, mice expressing all or only single isoforms of VEGF, known as Vegfa120/120, Vegfa164/164, and Vegfa188/188 mice were developed. Primary fibroblasts were isolated from mouse embryos that were produced by heterozygous breeding pairs of mice expressing single or all isoforms of vascular endothelial growth factor-A (VEGF-A) on Swiss background. Fibroblasts were immortalized and oncogenically transformed by retroviral transduction with SV40 and HRAS (characterised in Tozer et al., 2008. Cancer Res; 68: (7)). The original rationale for the development of these cell lines relates to the fact that tubulin-binding vascular-disrupting agents (VDA) are currently in clinical trials for cancer therapy but the factors that influence tumour susceptibility to these agents are poorly understood. Researchers evaluated the consequences of modifying tumour vascular morphology and function on vascular and therapeutic response to combretastatin-A4 3-O-phosphate (CA-4-P), which was chosen as a model VDA. The cell lines themselves could be potentially valuable for the commercial/pharmaceutical industry.
- Research area: Cancer; Cell Signaling & Signal Transduction; Developmental Biology; Drug Discovery & Development; Metabolism
- Production details: Using Cre/Lox technology, mice expressing all or only single isoforms of VEGF, known as Vegfa120/120, Vegfa164/164, and Vegfa188/188 mice were developed. Primary fibroblasts were isolated from mouse embryos that were produced by heterozygous breeding pairs of mice expressing single or all isoforms of vascular endothelial growth factor-A (VEGF-A) on Swiss background. Fibroblasts were immortalized and oncogenically transformed by retroviral transduction with SV40 and HRAS (characterised in Tozer et al., 2008. Cancer Res; 68: (7)).
- Cellosaurus ID: CVCL_HG20
- For Research Use Only
Target Details
- Target: VEGF120
Application Details
Handling
- Format: Frozen
- Growth medium: High glucose DMEM (Invitrogen) medium, L-glutamine, FCS, G-418 and puromycin. antibiotics G-418 and puromycin
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Tozer et al. 2008. Cancer Res. 68(7):2301-11. PMID: 18381437.
- • Blood vessel maturation and response to vascular-disrupting therapy in single vascular endothelial growth factor-A isoform-producing tumors.
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