fR2 Cell Line
Exclusive tool. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumorigenic in nude mice.
Contributors
| Inventor | Institute |
|---|---|
| Joyce Taylor-Papadimitriou ; Sidney Chang | Cancer Research UK, Lincoln's Inn Fields Institute |
| SKU: | 153246 |
|---|---|
| Product description: | . Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumorigenic in nude mice. |
| Gender: | Female |
| CRISPR: | Yes |
| Conditional: | No |
| Production details: | The breast epithelial cell line fR2 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40. |
| Additional notes: | STR-PCR Data: Amelogenin: X CSF1PO: 12,13 D13S317: 12 D16S539: 11,13 D5S818: 12,13 D7S820: 8,12 THO1: 9.3 TPOX: 8 vWA: 20 |
| Cellosaurus ID: | CVCL_2444 |
| Disease: | Cancer |
| Cat. #: | 153246 |
|---|---|
| Provenance and Ownership: | The fR2 Cell Line is owned by Cancer Research UK. CancerTools supplies the fR2 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing fR2 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | 10XUAS IVS p10 |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Research Fields: | Cancer; Drug development |
| Organism: | Human |
| Tissue: | Breast |
| Gender: | Female |
| Model: | Immortalised Line |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Breast cancer;Breast cancer |
| Primary citation: | Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290. |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37° C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10ug/ml insulin + 5ug/ml hydrocortisone + 10% FBS. |
| Mycoplasma free: | Yes |
| References: |
Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290. |
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