fR2 Cell Line
- Name Joyce Taylor-Papadimitriou ; Sidney Chang
- Institute Cancer Research UK, Lincoln's Inn Fields Institute
- Primary citation Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290.
Tool name: fR2 Cell Line
Tool type: Cell Lines
Cancer type: Breast cancer
Growth properties: Adherent
Model: Immortalised Line
Application: Investigating relationship between transformation and differentiation
Description: The fR2 Cell Line was established by infecting milk cultures with with simian vacuolating virus 40 (SV40). fR2 cells express Muc-1 (also known as HMFG-1 antigen), which is a mucin-like component of human milk fat globule membranes.
fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumourigenic in nude mice.
This cell line is positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, 16, 17 and 19. Although fR2 is morphologically different from fR5 cell line, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions.
This cell line could be useful for studying the relationship between transformation and differentiation in human mammary epithelial cells.
Research area: Cancer; Drug development
Production details: The breast epithelial cell line fR2 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40.
Cellosaurus ID: CVCL_2444
Additional notes: STR-PCR Data:
- For Research Use Only
- • Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290.