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Contributor Information

  • Name Joyce Taylor-Papadimitriou ; Sidney Chang
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: fR2 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Description: fR2 cells have been shown to express Muc-1, also known as HMFG-1 antigen, which is a mucin-like component of human milk fat globule membranes. They are positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, 16, 17 and 19. Although fR2 is morphologically different from fR5, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumorigenic in nude mice.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10 ?g/ml insulin + 5 ?g/ml hydrocortisone + 10% FBS
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.
  •   Establishment and characterization of SV40-transformed human breast epithelial cell lines.