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Contributor Information

  • Name Joyce Taylor-Papadimitriou ; Sidney Chang
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: fR5 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Description: fR5 cells do not express Muc-1, also known as HMFG-1 antigen, which is a mucin-like component of human milk fat globule membranes. They are negative for keratins 4, 6, 7, 8, 10, 13 14, 16, 17, 18 and 19. Although fR5 is morphologically different from fR2, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR5 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumourigenic in nude mice.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10Âľg/ml insulin + 5Âľg/ml hydrocortisone + 10% FBS
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.
  •   Establishment and characterization of SV40-transformed human breast epithelial cell lines.