Pr8/22 Cell Line

The cell line Pr8/22 has been derived from P388, a methylcholanthrene-induced lymphoid neoplasm originating in DBA/2 mouse and converted to ascitic form in the first mouse transfer. Pr8/22 is resistant to daunorubicin and is multi drug resistant (MDR). Cells should be treated with the drug at least once a month and before freezing, however after resuscitating daunorubicin should not be added until the first passage. It is advisable to keep a backup culture which is not drug-challenged.

Contributors

Inventor Institute
Timothy Ward Cancer Research UK Manchester Institute
Cat. #: 153248
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Drug development;Genetics
Organism: Mouse
Tissue: Lymphatic Tissue
Model: Tumour line
Growth properties: Suspension
Cancer Types In Detail: Drug resistant
Alternate name: Pr8/22; PR8/22; P388R
Product description: The cell line Pr8/22 also known as P388 Pr8/22 has been derived from P388 (an immortal murine acute leukaemia cell line). Pr8/22 is resistant to daunorubicin (anthracycline antibiotic) and is multi drug resistant (MDR). Cells should be treated with the drug at least once a month and before freezing, however after resuscitating daunorubicin should not be added until the first passage. It is advisable to keep a backup culture which is not drug-challenged. Pr8/22 proves valuable in studying mechanisms underlying anthracycline antibiotic resistance and for making predictive assessments of resistance patterns. 
Conditional: No
Production details: The cell line Pr8/22 has been derived from P388, a methylcholanthrene-induced lymphoid neoplasm.
Cellosaurus ID: CVCL_4276
Parental cell line: P388
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Sub-culture routine: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. It is recommended to culture the cells without drug upon resuscitation until the first passage.Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS); treat once a month with 0.1µM Daunorubicin.
Subculture routine: Cells should be treated with the drug at least once a month and before freezing, however after resuscitating daunorubicin should not be added until the first passage. It is advisable to keep a backup culture which is not drug-challenged.
References:

McGown et al. 1983. Cancer Chemother Pharmacol. 11(2):113-6. PMID: 6627597.

Comparative studies of the uptake of daunorubicin in sensitive and resistant P388 cell lines by flow cytometry and biochemical extraction procedures.

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