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Contributor Information

  • Name Timothy Ward
  • Institute Cancer Research UK Manchester Institute

Tool Details

  • Tool name: Pr8/22 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: P388
  • Organism: Mouse
  • Tissue: Lymphatic Tissue
  • Cancer type: Digestive / Gastrointestinal cancer
  • Disease: Cancer
  • Growth properties: Suspension
  • Model: Tumour line
  • Conditional: No
  • Description: The cell line Pr8/22 has been derived from P388, a methylcholanthrene-induced lymphoid neoplasm originating in DBA/2 mouse and converted to ascitic form in the first mouse transfer. Pr8/22 is resistant to daunorubicin and is multi drug resistant (MDR). Cells should be treated with the drug at least once a month and before freezing, however after resuscitating daunorubicin should not be added until the first passage. It is advisable to keep a backup culture which is not drug-challenged.
  • Research area: Cancer; Genetics; Drug development
  • Production details: The cell line Pr8/22 has been derived from P388, a methylcholanthrene-induced lymphoid neoplasm.
  • Cellosaurus ID: CVCL_4276

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Sub-culture routine: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37?‚°C. It is recommended to culture the cells without drug upon resuscitation until the first passage.Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS); treat once a month with 0.1?„?žM Daunorubicin.
  • Shipping conditions: Dry ice

Documentation

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References

  • •  McGown et al. 1983. Cancer Chemother Pharmacol. 11(2):113-6. PMID: 6627597.
  • •  Comparative studies of the uptake of daunorubicin in sensitive and resistant P388 cell lines by flow cytometry and biochemical extraction procedures.