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Contributor Information

  • Name Chris Marshall
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: 3.334 SC5/8 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: 3T3TK3 cells and normal rat kidney cells
  • Organism: Hybrid
  • Tissue: Fibroblast
  • Description: Hybrid between un-transformed mouse 3T3TK3 cells and normal rat kidney cells transformed with RSV. The hybrid cells express a non-transformed phenotype. The cells exhibit anchorage-independent growth at 35C but not at 39.5C, the non-permissive temperature and showed differences in microfilament bundle formation at both temperatures.
  • Research area: Adhesion ; Cell Structure and Motility

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 35°C; 5% CO2.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Marshall et al. 1980. Exp Cell Res. 127(2):373-84. PMID: 6247175.
  •   Suppression of the transformed phenotype with retention of the viral 'src' gene in cell hybrids between Rous sarcoma virus-transformed rat cells and untransformed mouse cells.