Cat. #153250
RT112/84 Cell Line
Cat. #: 153250
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Bladder
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Chris Marshall
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Primary Citation: Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: RT112/84 Cell Line
- Alternate name: RT112-84; RT-112/84; RT11284
- Cancer: Genitourinary cancer
- Cancers detailed: Human bladder carcinoma epithelial
- Research fields: Cancer;Drug development
- Organism: Human
- Tissue: Bladder
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Conditional: Yes
- Description: Human bladder transitional cell carcinoma cell line characterised by high E-cadherin expression and strong epithelial adhesion properties. Demonstrates limited invasive capacity under standard conditions, with invasion significantly enhanced by E-Cadherin disruption (via antibodies). A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies.
- Application: Tumourigenicity studies; studying cytotoxic effects of lipoic acid and calcium hydroxycitrate
- Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17
- Cellosaurus id: CVCL_2714
Applications
- Application: Tumourigenicity studies; studying cytotoxic effects of lipoic acid and calcium hydroxycitrate
- Application notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17
Handling
- Format: Frozen
- Growth medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent.
References
- Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752
- Davies et al. Anticancer Res. 1999 Jan-Feb 19(1A):547-52. PMID: 10226596
- Wang et al. Cancer Genet Cytogenet. 1995 Jul 15:82(2):170-4. PMID: 7664249
- Fujiyama et al. Br J Cancer. 2001 Feb:84(4):558-64. PMID: 11207054
- Rothenberg et al. Cancer Res. 2010 Mar 15:70(6):2158-64. Epub 2010 Mar 9. PMID: 20215515
- Awsare et al. Oncol Rep. 2011 Jun:25(6):1503-9. Epub 2011 Apr 4. PMID: 21468549
- Barretina et al. Nature. 2012 Mar 28:483(7391):603-7. Nature. 2012 Dec 13:492(7428):290. PMID: 22460905
- Weitzet al. Front Oncol. 2017 Aug 7:7:161. PMID: 28824873
- Zuiverloon et al. Bladder Cancer. 2018 Apr 26:4(2):169-183. PMID: 29732388
- Ghandi et al. Nature. 2019 May:569(7757):503:508. Epub 2019 May 8. PMID: 3106870
