RT112/84 Cell Line

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A patient-derived bladder cancer cell line used as a model of low-invasive disease to study urothelial differentiation, tumour invasion, and bladder cancer progression. Only available through CancerTools.

Contributors

Inventor Institute
Chris Marshall Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 153250
Product description: The RT112/84 cell line is derived from human bladder carcinoma. This cell line is a slow growing tumour which does not form colonies in soft agar. RT1112/84 cells requires 400 cells/dish to form colonies. RT112/84 is tumourigenic in nude mice. This cell line expresses high levels of E-cadherin, alpha-, beta-, and gamma-catenins.
Alternate name: RT112-84; RT-112/84; RT11284
Gender: Female
Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17
Cellosaurus ID: CVCL_2714
Disease: Cancer
Cat. #: 153250
Provenance and Ownership: The RT112/84 Cell Line is owned by Cancer Research UK. CancerTools supplies the RT112/84 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing RT112/84 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Cancer types: Bladder cancer
Organism: Human
Tissue: Bladder
Gender: Female
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Human bladder carcinoma epithelial
Primary citation: Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752.
Format: Frozen
Shipping conditions: Dry ice
Growth medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70-80%) seeding at 2-4×10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Subculture weekly – cells are difficult to trypsinise, and should be kept sub-confluent.
References:

Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752.

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RT112/84 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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