RT112/84 Cell Line

The RT112/84 cell line is derived from human bladder carcinoma.

Contributors

Inventor Institute
Chris Marshall Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 153250
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Bladder
Gender: Female
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Human bladder carcinoma epithelial
Primary citation: Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752.
Alternate name: RT112-84; RT-112/84; RT11284
Product description: The RT112/84 cell line is derived from human bladder carcinoma.
Gender: Female
Conditional: Yes
Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17
Cellosaurus ID: CVCL_2714
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70-80%) seeding at 2-4×10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Subculture weekly – cells are difficult to trypsinise, and should be kept sub-confluent.
References:

Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752.

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