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Contributor Information

  • Name Chris Marshall
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: RT112/84 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Bladder
  • Cancer type: Human bladder carcinoma epithelial
  • Disease: Cancer
  • Conditional: Yes
  • Description: A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Subculture Routine: Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent.Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752.