DOK Cell Line
- Name Sidney Chang
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool name: DOK Cell Line
Tool type: Cell Lines
Cancer type: Head and neck cancer
Growth properties: Adherent
Model: Tumour line
Description: The degree of dysplasia in the patient was described as mild to moderate. The cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene (codon 188-191) could be identified. DOK cells are non-tumourigenic in athymic nude mice.
Research area: Cancer; Drug development
Production details: The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the establishment of DOK.
Cellosaurus ID: CVCL_1180
Additional notes: STR-PCR Data:
- For Research Use Only
- • Burns et al. 1994. Br J Cancer. 70(4):591-5. PMID: 7917902.
- • The p53 status of cultured human premalignant oral keratinocytes.
- • Chang et al. 1992. Int J Cancer. 52(6):896-902. PMID: 1459732.
- • DOK, a cell line established from human dysplastic oral mucosa, shows a partially transformed non-malignant phenotype.