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Contributor Information

  • Name Sidney Chang
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: DOK Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Tongue
  • Cancer type: Human Caucasian dysplastic oral keratinocyte
  • Disease: Cancer
  • Conditional: Yes
  • Description: The degree of dysplasia in the patient was described as mild to moderate. The cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene (codon 188-191) could be identified. DOK cells are non-tumourigenic in athymic nude mice.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Subculture Routine: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Culture Medium: DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10% Foetal Bovine Serum (FBS).
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Burns et al. 1994. Br J Cancer. 70(4):591-5. PMID: 7917902.
  •   The p53 status of cultured human premalignant oral keratinocytes.
  •   Chang et al. 1992. Int J Cancer. 52(6):896-902. PMID: 1459732.
  •   DOK, a cell line established from human dysplastic oral mucosa, shows a partially transformed non-malignant phenotype.