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Contributor Information

  • Name Sidney Chang
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: DOK Cell Line
  • Tool type: Cell Lines
  • Organism: Human
  • Tissue: Tongue
  • Cancer type: Head and neck cancer
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: Yes
  • Description: The degree of dysplasia in the patient was described as mild to moderate. The cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene (codon 188-191) could be identified. DOK cells are non-tumourigenic in athymic nude mice.
  • Research area: Cancer; Drug development
  • Production details: The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the establishment of DOK.
  • Cellosaurus ID: CVCL_1180
  • Additional notes: STR-PCR Data: Amelogenin: X,Y CSF1PO: 12 D13S317: 11,13 D16S539: 9,12 D5S818: 12 D7S820: 11,13 THO1: 6,8 TPOX: 10 vWA: 14,18

  • For Research Use Only

Target Details

Application Details

  • Application notes: STR-PCR Data: Amelogenin: X,Y CSF1PO: 12 D13S317: 11,13 D16S539: 9,12 D5S818: 12 D7S820: 11,13 THO1: 6,8 TPOX: 10 vWA: 14,18

Handling

  • Format: Frozen
  • Growth medium: Subculture Routine: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm?‹Â› using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37?‚°C.Culture Medium: DMEM + 2mM Glutamine + 5?„?žg/ml Hydrocortisone + 10% Foetal Bovine Serum (FBS).
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Burns et al. 1994. Br J Cancer. 70(4):591-5. PMID: 7917902.
  • •  The p53 status of cultured human premalignant oral keratinocytes.
  • •  Chang et al. 1992. Int J Cancer. 52(6):896-902. PMID: 1459732.
  • •  DOK, a cell line established from human dysplastic oral mucosa, shows a partially transformed non-malignant phenotype.