K562 AZQR Cell Line

K562 AZQ was developed from the parent K562 cell line by treatment with AZQ C215-bis (carboethoxyamino)-3, 6-diazitinyl 1;4 (benzoquinone).

Contributors

Inventor Institute
Timothy Ward Cancer Research UK Manchester Institute
Cat. #: 153252
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Blood cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Lymphatic Tissue
Model: Tumour line
Growth properties: Suspension
Cancer Types In Detail: Human caucasian chronic myelogenous leukemia (drug resistant)
Product description: The cells have been shown to contain decreased levels of glutathione and superoxoid dismutase. They are cross-resistant to adriamycin, mitzolamide, MMNG and mitmycin. Cells should be challenged with AZQ at every 4th passage at a minimum density of 100,000 cells/ml. It is recommended to cultivate the cells without drug on resuscitation.
Conditional: No
Production details: The drug resistant cell line K562 AZQ was developed from the parent K562 cell line by treatment with AZQ C215-bis (carboethoxyamino)-3, 6-diazitinyl 1;4 (benzoquinone).
Cellosaurus ID: CVCL_2967
Parental cell line: K562
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Subculture Routine: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37?‚°C.Culture Medium: RPMI 1640 + 10% Foetal Bovine Serum (FBS) + 2mM Glutamine. Challenge with 1 mM AZQ every 4th passage.
References:

Ward et al. 1995. Biochem Pharmacol. 50(4):459-64. PMID: 7646550.

Cross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones.

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