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Contributor Information

  • Name Timothy Ward
  • Institute Cancer Research UK Manchester Institute

Tool Details

  • Tool name: K562 AZQR Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: K562
  • Organism: Human
  • Tissue: Lymphatic Tissue
  • Cancer type: Human caucasian chronic myelogenous leukemia (drug resistant)
  • Disease: Cancer
  • Description: The cells have been shown to contain decreased levels of glutathione and superoxoid dismutase. They are cross-resistant to adriamycin, mitzolamide, MMNG and mitmycin. Cells should be challenged with AZQ at every 4th passage at a minimum density of 100,000 cells/ml. It is recommended to cultivate the cells without drug on resuscitation.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Subculture Routine: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C.Culture Medium: RPMI 1640 + 10% Foetal Bovine Serum (FBS) + 2mM Glutamine. Challenge with 1 mM AZQ every 4th passage.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Ward et al. 1995. Biochem Pharmacol. 50(4):459-64. PMID: 7646550.
  •   Cross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones.