CELL LINES
Contributor Information
- Name Majlinda Lako ; Joseph Collin
- Institute Newcastle University
Tool Details
- Tool name: H9 hESC EGFP-Cone-Rod homeobox Cell Line
- Alternate names: H9 CRX-GFP, hESC, Human embryonic stem cells, CRX,Orthodenticle Homeobox, CORD2, Cone-Rod Homeobox Protein, LCA7 3, OTX3, CRD, AMD,
- Tool type: Cell Lines
- Parental cell line: H9 hESC
- Organism: Human
- Tissue: Embryo
- Disease: Retinal development & age-related macular degeneration
- Model: Reporter
- Conditional: No
- Description: A frequent cause of vision impairment and blindness associated with inherited retinal diseases and age related macular degeneration, which causes blurred or an absence of vision in the center of the visual field, is due to degeneration of retinal pigmented epithelium and photoreceptors, Replacement with stem cell derived equivalents is an excellent approach to try and preserve the retinal structures, function and vision. The Cone-Rod Homeobox gene is a key transcription factor involved in retinal development. CRX is known to be expressed in postmitotic retinal photoreceptors, and to play a key role in photoreceptor formation and maturation. This human embryonic stem cell line encodes a cone-rod homeobox gene fused at the 3' end to eGFP and has been fully characterized. This research tool enables researchers to mimic the expression of the CRX gene and to assess and investigate these cells offering pluripotent stem cell differentiation.
- Research area: Developmental Biology; Gene Expression; Genetic Studies Tools
- Production details: Differentiation to 3D ocular-like structures which contain a retinal pigment epithelium, neural retina, primitive lens and corneal like structures is achieved by culture in low attachment plates with ventral neural induction media supplemented with recombinant human IGF-1 (5ng/ml) for 37 days, then in basal knockout serum free media with 10ng/ml IGF-1 until day 90
- For Research Use Only
Target Details
- Target: Cone-Rod homeobox
Application Details
Handling
- Format: Frozen
- Growth medium: Feeder-free mTeSR1 media, with Matrigel-coated plates
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
- Available on request
References
- • PMID: 26608863 PMID: 25827910
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