HR1 Cell Line
- Name Simon Cook
- Institute Babraham Institute
Tool name: HR1 Cell Line
Alternate names: RAF proto-oncogene serine/threonine-protein kinase (EC:126.96.36.199), Proto-oncogene c-RAF, cRaf, Raf-1
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: HEK 293
Disease: Cancer; Inflammation
Growth properties: Adherent cell line
Conditional description: Stable transfectant, conditional functionality: c-Raf1 kinase activity and ERK1/2 signalling pathway activated following 4-hydroxytamoxifen (4-HT) treatment of cells.
Description: HR1 cells are HEK 293 cells stably expressing conditional kinase Raf-1:ER* from the pCMV Neo Myc plasmid. Raf-1:ER* (also known as CRAF:ER*) consists of the isolated kinase domain of c-Raf1 fused in-frame to a modified form of the hormone binding domain of the estrogen receptor (hbER*) that can be de-repressed by 4-hydroxytamoxifen (4-HT) but not ?-estradiol. In this case the * refers to a point mutation that ablates estradiol binding but allows 4-HT binding.
Activation of Raf-1:ER* leads to the selective activation of the ERK1/2 pathway. This cell line can be used to study the cellular role and factors impacting on the ERK1/2 signalling pathway such as gene expression, cell proliferation, cell cycle arrest and cell death. The cells are G418 resistant. Conditional kinase activation of Raf-1:ER* can be induced with 100nM 4-HT.
Research area: Cancer; Apoptosis and autophagy; Cell biology; Cell signaling and signal transduction; Immunology
Production details: HEK 293 cells were transfected with the pCMV Neo Myc plasmid expressing ÄË?Â?ÂRaf-1:ER*. Stable transfectants were selected by neomycin resistance using G418 (Geneticin) and ring cloning.
Cellosaurus ID: CVCL_2676
- For Research Use Only
- • Gilley et al. 2009. Cell Signal. 21(6):969-77. PMID: 19249353.
- • ERK1/2, but not ERK5, is necessary and sufficient for phosphorylation and activation of c-Fos.
- • Wiggins et al. 2007. Cell Signal. 19(12):2605-11. PMID: 17884340.
- • c-Cbl is not required for ERK1/2-dependent degradation of BimEL.
- • Ewings et al. 2007. EMBO J. 26(12):2856-67. PMID: 17525735.
- • ERK1/2-dependent phosphorylation of BimEL promotes its rapid dissociation from Mcl-1 and Bcl-xL.
- • Boughan et al. 2006. J Biol Chem. 281(17):11637-48. PMID: 16513653.
- • Nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: critical regulators of beta-defensins during Helicobacter pylori infection.