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Contributor Information

  • Name Leonard Franks
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: MB49 Mouse Bladder Carcinoma Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Primary
  • Parental cell line:
  • Organism: Mouse
  • Tissue: Bladder
  • Cancer type: Urinary bladder neoplasms, bladder carcinoma
  • Disease: Cancer
  • Description: MB49 cell line used as an in vitro and in vivo model of bladder cancer, with enhanced metastatic potential for further migratory investigation. Background and Research Application MB49 cells are a urothelial carcinoma line derived from an adult C57BL/6 mouse by exposure of primary bladder epithelial cell explants to 7,12-dimethylbenz[a]anthracene (DMBA) for 24 hr followed by long-term culture. The syngeneic, murine model of bladder cancer has been widely used for more than 35 years. MB49 cell line was created to demonstrate the effects of ageing on neoplastic transformation in long term primary cultures of the bladder. There is a high age-associated bladder tumour incidence in men. The donor mouse was male, however, a 2012 karyotypic analysis (Fabris et al., 2012. Cancer Genet.) found that the MB49 tumour cell line has lost the Y-chromosome and therefore does not express male-specific antigens. A more recent study evaluated the cell lines sensitivity to testosterone and the pregnancy hormone, human chorionic gonadotropin (hCG). White-Gilbertson et al., 2016 discovered that MB49 tumour growth was significantly greater in male mice than female mice and that pregnancy did not affect the size or rate MB49 tumour growth in female mice. MB49 cells failed to proliferate in response to hCG in vitro and the functional receptor for gonadotropins was absent. Dihydrotestosterone strongly stimulated growth of MB49 cells in vitro.
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details


  • Format: Frozen
  • Growth medium: DMEM, FBS. Note that these cells do not form a 100% confluent monolayer but at 70% confluence tend to detach in small clumps that float in the media. About 10-20% of the cells will be attached with a spindle-like epithelial morphology, the remainder will appear rounded
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1


  • Available on request


  •   Albert et al. 2019. Oncol Lett. 17(3):3141-3150. PMID: 30867744.
  •   Plote et al. 2019. Oncoimmunology. 8(5):e1577125. PMID: 31069136.
  •   Shi et al. 2019. Onco Targets Ther. 12:4403-4413. PMID: 31239709.
  •   Sex differences in the MB49 syngeneic, murine model of bladder cancer.
  •   White-Gilbertson et al. 2016. Bladder (San Franc). 3(1):. PMID: 26998503.
  •   Kasman et al. 2013. J Vis Exp. (82):50181. PMID: 24326612.
  •   An orthotopic bladder cancer model for gene delivery studies.
  •   Zhu et al. 2013. BMC Urol. 13:57. PMID: 24188098.
  •   A modified method for isolation of bladder cancer stem cells from a MB49 murine cell line.
  •   Fabris et al. 2012. Cancer Genet. 205(4):168-76. PMID: 22559978.
  •   Cytogenetic characterization of the murine bladder cancer model MB49 and the derived invasive lineMB49-I.
  •   Chen et al. 2009. J Urol. 182(6):2932-7. PMID: 19853870.
  •   MB49 murine urothelial carcinoma: molecular and phenotypic comparison to human cell lines as a model of the direct tumor response to bacillus Calmette-Guerin.
  •   Loskog et al. 2005. Lab Anim. 39(4):384-93. PMID: 16197705.
  •   Optimization of the MB49 mouse bladder cancer model for adenoviral gene therapy.
  •   Brocks et al. 2005. J Urol. 174(3):1115-8. PMID: 16094076.
  •   Inhibition of tumor implantation by intravesical gemcitabine in a murine model of superficial bladder cancer.
  •   Gnther et al. 1999. Cancer Res. 59(12):2834-7. PMID: 10383142.
  •   Optimizing syngeneic orthotopic murine bladder cancer (MB49).
  •   Summerhayes et al. 1979. J Natl Cancer Inst. 62(4):1017-23. PMID: 107359.
  •   Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro.