MB49 Mouse Bladder Carcinoma Cell Line
- Name Leonard Franks
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool name: MB49 Mouse Bladder Carcinoma Cell Line
Tool type: Cell Lines
Tool sub-type: Primary
Cancer type: Genitourinary cancer
Model: Tumour line
Description: MB49 cell line used as an in vitro and in vivo model of bladder cancer, with enhanced metastatic potential for further migratory investigation.
Background and Research Application
MB49 cells are a urothelial carcinoma line derived from an adult C57BL/6 mouse by exposure of primary bladder epithelial cell explants to 7,12-dimethylbenz[a]anthracene (DMBA) for 24 hr followed by long-term culture. The syngeneic, murine model of bladder cancer has been widely used for more than 35 years. MB49 cell line was created to demonstrate the effects of ageing on neoplastic transformation in long term primary cultures of the bladder. There is a high age-associated bladder tumour incidence in men.
The donor mouse was male, however, a 2012 karyotypic analysis (Fabris et al., 2012. Cancer Genet.) found that the MB49 tumour cell line has lost the Y-chromosome and therefore does not express male-specific antigens. A more recent study evaluated the cell lines sensitivity to testosterone and the pregnancy hormone, human chorionic gonadotropin (hCG). White-Gilbertson et al., 2016 discovered that MB49 tumour growth was significantly greater in male mice than female mice and that pregnancy did not affect the size or rate MB49 tumour growth in female mice. MB49 cells failed to proliferate in response to hCG in vitro and the functional receptor for gonadotropins was absent. Dihydrotestosterone strongly stimulated growth of MB49 cells in vitro.
Research area: Cancer
Implanted long term MB49 cells were subcutaneously injected into mice, with the primary tumours resected, mechanically disrupted and trypsin digested to obtain single cells, most of which were adherent but a small population of spheroidal 3D structures in culture. These are likely stem like, basal and highly metastatic, which is ideal for researching metastatic urothelial carcinoma.
Immortalisation process: 7,12-dimethylbenz[a]anthracene on day 2 of culture.
Cellosaurus ID: CVCL_7076
- For Research Use Only
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- • Shi et al. 2019. Onco Targets Ther. 12:4403-4413. PMID: 31239709.
- • Sex differences in the MB49 syngeneic, murine model of bladder cancer.
- • White-Gilbertson et al. 2016. Bladder (San Franc). 3(1):. PMID: 26998503.
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- • An orthotopic bladder cancer model for gene delivery studies.
- • Zhu et al. 2013. BMC Urol. 13:57. PMID: 24188098.
- • A modified method for isolation of bladder cancer stem cells from a MB49 murine cell line.
- • Fabris et al. 2012. Cancer Genet. 205(4):168-76. PMID: 22559978.
- • Cytogenetic characterization of the murine bladder cancer model MB49 and the derived invasive lineMB49-I.
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- • MB49 murine urothelial carcinoma: molecular and phenotypic comparison to human cell lines as a model of the direct tumor response to bacillus Calmette-Guerin.
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- • Optimization of the MB49 mouse bladder cancer model for adenoviral gene therapy.
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- • Optimizing syngeneic orthotopic murine bladder cancer (MB49).
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- • Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro.