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Contributor Information

  • Name Stephen Prime
  • Institute University of Bristol

Tool Details

  • Tool name: H157 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Tissue: Buccal mucosa
  • Disease: Cancer
  • Conditional: Yes
  • Description: Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a male patient, age 84. STNMP stage II, well differentiated, node positive tumour. Mutant p53, codon 306 exon 8, G to A; wild type K-, N- and Ha-ras. Non-tumourigenic in athymic nude mice by subcutaneous injection and on injection into the floor of the mouth (orthotopic), but form epidermoid cysts subcutaneously
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:8 to 1:10 5% CO2; 37°C. Suggested seeding density 5 x 1000 cells/cm². DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
  •   Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
  •   Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
  •   Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.
  •   Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579.
  •   Ras gene point mutation is a rare event in premalignant tissues and malignant cells and tissues from oral mucosal lesions.
  •   Prime et al. 1990. J Pathol. 160(3):259-69. PMID: 1692339.
  •   The behaviour of human oral squamous cell carcinoma in cell culture.