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Contributor Information

  • Name Stephen Prime
  • Institute University of Bristol

Tool Details

  • Tool name: H314 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Tissue: Oral Cavity
  • Disease: Cancer
  • Conditional: Yes
  • Description: Established from a squamous cell carcinoma (SCC) of the floor of the mouth (20mm-40mm) of an 82 year-old male patient. STNMP stage II, moderately differentiated, node positive tumour. Mutant p53, codon 176 exon 5, G to T and 373 exon 11, A to G; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice on subcutaneous injection; on injection into the floor of the mouth, cells spread to lymphatics and lymph nodes. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%), approximately every 6-7 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 7 x 1000 cells/cm². DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
  •   Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
  •   Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
  •   TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.
  •   Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
  •   Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.
  •   Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579.
  •   Ras gene point mutation is a rare event in premalignant tissues and malignant cells and tissues from oral mucosal lesions.
  •   Prime et al. 1990. J Pathol. 160(3):259-69. PMID: 1692339.
  •   The behaviour of human oral squamous cell carcinoma in cell culture.