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Contributor Information

  • Name Stephen Prime
  • Institute University of Bristol
  • Primary citation Prime et.al. 1990. J Pathol. 160(3):259-69. PMID: 1692339

Tool Details

  • Tool name: H400 Cell Line
  • Alternate names: H400
  • Tool type: Cell Lines
  • Organism: Human
  • Donor: Female, 55 y.o. Clinical features: site: alveolar process; size (mm): 20-40; nodes: - ; metastases: - ; pathology: moderately differentiated; grade: II
  • Tissue: The alveolar process of the maxilla
  • Gender: Female
  • Cancer type: Head and neck cancer
  • Growth properties: Adherent
  • Model: Mutant; Tumour line
  • Model description: Mutant p53, codon 283 exon 8, C to G wild type K-, N- and Ha-ras. Non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth.
  • CRISPR: No
  • Conditional: No
  • Application: Disease modeling, malignant progression studies, gene mutation and expression analysis
  • Description: Established from a squamous cell carcinoma (SCC) of the alveolar process (20mm - 40mm) of a female patient aged 55. STNMP stage II, moderately differentiated, node negative tumour. This cell line is highly responsive to TGF-beta. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth
  • Research area: Cancer
  • Cellosaurus ID: CVCL_2464
  • Additional notes: Haplotype information: A*11,A*29; B*07,B*15; Cw*03,Cw*15

  • For Research Use Only

Target Details

Application Details

  • Application: Disease modeling, malignant progression studies, gene mutation and expression analysis

Handling

  • Format: Frozen
  • Growth medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
  • Temperature: 37C
  • Atmosphere: 5% CO2
  • Volume: 1 ml
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Subculture routine: Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37C. Suggested seeding density 5 x 1000 cells/cm2. Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability

Documentation

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References

  • •  Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286
  • •  Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893
  • •  Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215
  • •  Yeudall et.al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579
  • •  Prime et.al. 1990. J Pathol. 160(3):259-69. PMID: 1692339