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Contributor Information

  • Name Stephen Prime
  • Institute University of Bristol

Tool Details

  • Tool name: H413 Cell Line
  • Tool type: Cell Lines
  • Tissue: Buccal mucosa
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Description: Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a 53 year-old female patient. STNMP stage II, moderately differentiated, node negative tumour. Mutant p53, codon 68 exon 4, G to T; wild type K-, N- and Ha-ras. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth
  • Research area: Cancer
  • Cellosaurus ID: CVCL_2465

  • For Research Use Only

Target Details

  • Target: Human oral squamous cell carcinoma, buccal mucosa

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37?‚°C. Suggested seeding density 5 x 1000 cells/cm?‹Â› . Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
  • •  Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
  • •  Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
  • •  TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.
  • •  Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
  • •  Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.
  • •  Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579.
  • •  Ras gene point mutation is a rare event in premalignant tissues and malignant cells and tissues from oral mucosal lesions.
  • •  Prime et al. 1990. J Pathol. 160(3):259-69. PMID: 1692339.
  • •  The behaviour of human oral squamous cell carcinoma in cell culture.