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Contributor Information

  • Name Fiona Watt
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: VP Cell Line
  • Alternate names: HPV, Human papillomavirus
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Human epidermal keratinocytes - strain V
  • Organism: Human
  • Tissue: Skin
  • Cancer type: HPV, carincogenesis, cervical, skin
  • Disease: Cancer
  • Description: The paired normal and immortalised cell lines provide a good model system for studying the interaction of the HPV genome with human cells and offer the potential for further studies on the effects of carcinogenesis and oncogenes in malignant conversion of HPV16-infected keratinocytes vp HPV16-immortalised keratinocyte cell line
  • Research area: Cancer ; Cell Cycle ; DNA Damage and Repair ; Drug Discovery & Development ; Immunology

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Composed of a 1+3 mixture of Ham’s F12 and DMEM, supplemented with 1.8x10-4M adenine, 10 % FCS, 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ng/ml EGF and 10-10M cholera toxin. Cultures should be maintained at 37oC, and medium changed every 2-3 days
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Hodivala et al. 1994. Oncogene. 9(3):943-8. PMID: 8108139.
  •   Integrin expression and function in HPV 16-immortalised human keratinocytes in the presence or absence of v-Ha-ras. Comparison with cervical intraepithelial neoplasia.
  •   Pei et al. 1991. Carcinogenesis. 12(2):277-84. PMID: 1847319.
  •   Two strains of human keratinocytes transfected with HPV16 DNA: comparison with the normal parental cells.