CELL LINES
Contributor Information
- Name Fiona Watt
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: VP Cell Line
- Alternate names: HPV, Human papillomavirus
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: Human epidermal keratinocytes - strain V
- Organism: Human
- Tissue: Skin
- Cancer type: HPV; Carincogenesis; Cervical; Skin
- Disease: Cancer
- Model: Immortalised Line
- Conditional: No
- Description: The paired normal and immortalised cell lines provide a good model system for studying the interaction of the HPV genome with human cells and offer the potential for further studies on the effects of carcinogenesis and oncogenes in malignant conversion of HPV16-infected keratinocytes vp HPV16-immortalised keratinocyte cell line
- Research area: Cancer; Cell Cycle; DNA Damage and Repair; Drug Discovery & Development; Immunology
- Production details: Primary human keratinocytes from newborn foreskin samples were used as the source of cells. These cells were then grown in culture and at passage 2 transfected with the pSV2neo/16 plasmid which contains the entire HPV16 genome.
- For Research Use Only
Target Details
- Target: HPV transfected human keratinocytes
Application Details
Handling
- Format: Frozen
- Growth medium: Composed of a 1+3 mixture of HamĂÂs F12 and DMEM, supplemented with 1.8x10-4M adenine, 10 % FCS, 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ng/ml EGF and 10-10M cholera toxin. Cultures should be maintained at 37oC, and medium changed every 2-3 days
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Hodivala et al. 1994. Oncogene. 9(3):943-8. PMID: 8108139.
- • Integrin expression and function in HPV 16-immortalised human keratinocytes in the presence or absence of v-Ha-ras. Comparison with cervical intraepithelial neoplasia.
- • Pei et al. 1991. Carcinogenesis. 12(2):277-84. PMID: 1847319.
- • Two strains of human keratinocytes transfected with HPV16 DNA: comparison with the normal parental cells.
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