Cat. #153460
SG01 Cell Line
Cat. #: 153460
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Subglottis
Disease: Cancer
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jason Powell ; Chris Ward
Institute: Newcastle University
Tool Details
*FOR RESEARCH USE ONLY
- Name: SG01 Cell Line
- Tool sub type: Continuous
- Parental cell: Subglottic brushing specimen
- Organism: Human
- Tissue: Subglottis
- Disease: Cancer
- Growth properties: Adherent
- Model: Immortalised Line
- Conditional: No
- Description: The subglottic airway is an important gatekeeper that functions to prevent lower lung infection and is a crucial area in terms of inflammation (subglottic stenosis) and malignancy.
- Production details: The SG01 Cell Line was derived from a single patients neoplasia-free human subglottic brushing specimen. The cells were immortalised using a SV40 Cell immortalization system, utilizing a recombinant lentiviral vector. SG01 were successfully lifted onto an air-liquid interface (ALI) culture system, using the appropriate growth medium. At ALI cells demonstrated cilia coverage and mucus production. Cells demonstrate tight epithelial junctions and relevant ion channels expression, including ENaC,...
Applications
- Application notes: How to plate the cells from frozen ampule: Prepare a flask coated with 10ug/cm2 Collagen (Type 1) at the following volumes 2.5mls/25cm2; 7.5ml/75cm2 and 17.25mls/175cm2. (Sigma 0.1% solution, 8919-20ML), diluted 1:10 in growth medium (BEGM). Add mixture at ratio of 0.1 ml per cm surface area. Incubate at 37?°C for approximately 6 hours and 5% CO2, or overnight at 4C. Remove excess fluid prior to use and wash flask with either PBS or media. Seeding density at first seed and...
Handling
- Format: Frozen
- Growth medium: SG01 cells were cultured on a collagen coated container in Bronchial Epithelial Cell Growth Medium (BEGM) (available from Clonetics Corporation, CC3171 (BEBM) plus additives CC4175 or BEGM Bullet Kit, CC3170). , supplemented with 100 g/mL streptomycin and 100 U/mL penicillin at 37?°C and 5% CO2. Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.05% trypsin/EDTA.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Establishment of an immortalized human subglottic epithelial cell line
- A Human in Vitro Model of the Subglottic Airway
