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Contributor Information

  • Name Jonathon Pines
  • Institute University of Cambridge

Tool Details

  • Tool name: Ruby-Mad2 Reporter Cell Line
  • Tool type: Cell Lines
  • Parental cell line: RPE1
  • Organism: Human
  • Tissue: Eye
  • Model: Reporter
  • Conditional: Yes
  • Description: RPE1 cell line in which the Ruby fluorescent protein has been knocked into one allele of the Mad2 gene.
  • Research area: Cell biology

  • For Research Use Only

Target Details

  • Target: Mad2

Application Details

Handling

  • Format: Frozen
  • Growth medium: F12/DMEM (Sigma: D6241) + 2mM GlutaMAX ?‚™ (Invitrogen), 10% Foetal Bovine Serum (FBS) + 0.348% sodium bicarbonate; 37oC; 5% CO2.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Collin et al. 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242.