CELL LINES
Contributor Information
- Name Richard Edmondson
- Institute Northern Institute For Cancer Research, Newcastle University
Tool Details
- Tool name: NUOC-1 Cell Line
- Alternate names: HGSC, high grade mixed ovarian cancer, epithelial ovarian cancer
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: Ascites of chemotherapy naive patient
- Organism: Human
- Tissue: Ovary
- Cancer type: High Grade Ovarian
- Disease: Cancer
- Growth properties: Doubling time approximately 58 hours
- Model: Cancer Model
- Conditional: No
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Description: Epithelial ovarian cancer is diagnosed at late disease stage in more than 70% of patients. Ovarian cancer is the leading cause of gynaecological cancer mortality worldwide with a 5-year overall survival of 3039%. It has long been recognised by clinicians that ovarian cancer is a set of heterogeneous diseases. NUOC-1 cell line represents a novel ovarian cancer cell line derived from the ascites of a chemotherapy na
ve patient with a primary mixed endometrioid/ clear cell/ high grade serous ovarian cancer. NUOC-1 cells grow as an adherent monolayer. The cells are TP53 wildtype, positive for PTEN, HER2 and HER3 expression but negative for oestrogen, progesterone and androgen receptor expression. NUOC-1 cells are competent in homologous recombination and non-homologous end joining, but base excision repair defective. Karyotype analysis demonstrated a complex tetraploid karyotype - Research area: Cancer; Drug Discovery & Development
- Production details: Ascites were collected from patients undergoing surgery for ovarian cancer. 20 ml of ascites was added to 20 ml of warmed culture medium in T75 flask, and incubated at 37ÄÂĂ°C, 5% CO2, 95% humidified air. The medium was aspirated and 13 ml of warmed fresh medium was replaced on day 3 to 5. The medium was replaced every 4 to 5 days until the cells approached confluence
- For Research Use Only
Target Details
- Target: Ovarian cancer
Application Details
- Application notes: NUOC-1 cells grow as an adherent monolayer. The cells are TP53 wildtype, positive for PTEN, HER2 and HER3 expression but negative for oestrogen, progesterone and androgen receptor expression. NUOC-1 cells are competent in homologous recombination and non-homologous end joining, but base excision repair defective. Karyotype analysis demonstrated a complex tetraploid karyotype
Handling
- Format: Frozen
- Growth medium: RPMI 1640 medium supplemented with 20% FCS, 20 mM L-glutamine and 1% penicillin and streptomycin
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
- Available on request
Related Tools
References
- • McCormick et al. 2017. Oncotarget. 8(16):26832-26844. PMID: 28460465.
- • Functional characterisation of a novel ovarian cancer cell line, NUOC-1.
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