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Contributor Information

  • Name Carlos Caldas
  • Institute Cancer Research UK Cambridge Institute

Tool Details

  • Tool name: HCT 116 BRCA2 -/- [46] Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Cancer type: Colorectal carcinoma
  • Disease: Cancer
  • Description: Homozygous knockout cell line used to investigate the effects of BRCA2 mutations in a range of cancers. Background and Research Application BRCA2 -/- HCT116 human colorectal carcinoma cells were generated to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. This includes the upregulation and effect of interferon-related genes (including APOBEC3F and APOBEC3G) when the BRAC2 gene is lost. This cell line shows phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors. BRCA2 also has a role in transcriptional activation. BRAC2 inherited mutations, predispose carriers to various early onset cancers, including breast and ovarian.
  • Research area: Cancer ; DNA Damage and Repair ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium:
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256.
  •   Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells.
  •   Issaeva et al. 2010. Cancer Res. 70(15):6268-76. PMID: 20631063.
  •   Issaeva et al. 2010. Cancer Res. 70(15):6268-76. PMID: 20631063.