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Contributor Information

  • Name Robert Lahue
  • Institute National University of Ireland Galway ; Brown University

Tool Details

  • Tool name: Msh3-/- SVG-A Cell Line
  • Alternate names: DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, DUP, Mismatch repair protein 1, MRP1
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: SVG-A immortalized human astrocytes
  • Organism: Human
  • Tissue: Brain
  • Disease: Neurodegenerative disease such as Huntington's
  • Application: Applications tested: DNA repair assays Microsatellite instability Trinucleotide expansion assay Cell background for rescue with wild type or variant Msh3 clones Immunoprecipitation control for Msh2-Msh3 protein complex Functional analysis of Msh3 variants
  • Description: The MSH3 gene encodes a DNA mismatch repair protein important in certain cancer types and in certain neurodegenerative diseases. Exon 2 of the MSH3 gene was targeted by CRISPR/Cas9. The resulting Msh3-/- cell line encodes a 3 amino acid deletion in Msh3 that results in 98% loss of Msh3 protein, as judged by western blots with two different antibodies. While ~98% of Msh3 protein is lost in these cells, the abundance of the related proteins Msh2 and Msh6 appear unaffected. This is relevant to DNA mismatch repair.
  • Research area: Cancer ; Cell Cycle ; DNA Damage and Repair
  • Additional notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license

  • For Research Use Only

Target Details

Application Details

  • Application: Applications tested: DNA repair assays Microsatellite instability Trinucleotide expansion assay Cell background for rescue with wild type or variant Msh3 clones Immunoprecipitation control for Msh2-Msh3 protein complex Functional analysis of Msh3 variants

Handling

  • Format: Frozen
  • Growth medium: DMEM supplemented with 10% FBS
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Keogh et al. 2017. Nucleic Acids Res. 45(17):10068-10078. PMID: 28973443.
  •   MutS? abundance and Msh3 ATP hydrolysis activity are important drivers of CTGCAG repeat expansions.