Msh3-/- SVG-A Cell Line
- Name Robert Lahue
- Institute National University of Ireland Galway ; Brown University
Tool name: Msh3-/- SVG-A Cell Line
Alternate names: DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, DUP, Mismatch repair protein 1, MRP1
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: SVG-A immortalized human astrocytes
Disease: Neurodegenerative disease such as Huntington's
Application: Applications tested:
DNA repair assays
Trinucleotide expansion assay
Cell background for rescue with wild type or variant Msh3 clones
Immunoprecipitation control for Msh2-Msh3 protein complex
Functional analysis of Msh3 variants
Description: The MSH3 gene encodes a DNA mismatch repair protein important in certain cancer types and in certain neurodegenerative diseases.
Exon 2 of the MSH3 gene was targeted by CRISPR/Cas9. The resulting Msh3-/- cell line encodes a 3 amino acid deletion in Msh3 that results in 98% loss of Msh3 protein, as judged by western blots with two different antibodies.
While ~98% of Msh3 protein is lost in these cells, the abundance of the related proteins Msh2 and Msh6 appear unaffected. This is relevant to DNA mismatch repair.
Research area: Cancer ; Cell Cycle ; DNA Damage and Repair
Additional notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license
- For Research Use Only
- • Keogh et al. 2017. Nucleic Acids Res. 45(17):10068-10078. PMID: 28973443.
- • MutS? abundance and Msh3 ATP hydrolysis activity are important drivers of CTGCAG repeat expansions.