BMI-1 4 Cell Line
- Name Ian Sayers
- Institute University of Nottingham
Tool name: BMI-1 4 Cell Line
Alternate names: Polycomb complex protein BMI-1, polycomb group RING finger protein 4; PCGF4; RING finger protein 51; RNF51; B cell-specific Moloney murine leukemia virus integration site 1; BMI-1; FLVI2/PCGF4; Mo-MLV; Moloney murine leukemia virus; NHBEC; Normal Human Bronchial Epithelial Cells; pLVX-BMI-1 Cell Line: COPD, ALI; airâ€“liquid interface
Tool type: Cell Lines
Tool sub-type: Primary
Parental cell line:
Disease: Respiratory disease
Description: Primary Human bronchial epithelial cells when grown in vitro have a limited lifespan and begin to deviate both in phenotype and morphology, losing the plasticity required around passage 4 or 5, for air-liquid interface (ALI) differentiation. These Human bronchial epithelial cells expressing BMI-1 retain both viability and differentiation potential of wild-type human bronchial epithelium while importantly not demonstrating changes in cell karyotype.
B lymphoma Moloney murine leukemia virus insertion region 1 homolog (BMI-1) is an oncogene which functions by regulating P16 and P19 cell cycle inhibitor genes and is also associated with erythroplakia and tongue cancer. BMI-1 is thought to repress, p16(Ink4a), a cyclin-dependent kinase inhibitor and tumor suppressor that induces cell cycle arrest at the Gap 1 phase. BMI-1 can therefore be used to delay cell senescence.
The airway epithelium is a critical interface acting as a barrier to potential pathogens and extraneous particles, assisting in regulation of host defense mechanisms like the inflammatory response.
Research area: Cell Cycle ; Cell Signaling & Signal Transduction ; Cell Structure and Motility ; Drug Discovery & Development ; Genetic Studies Tools
- For Research Use Only
- • Torr et al. 2016. Physiol Rep. 4(16):. PMID: 27558999.
- • Expression of polycomb protein BMI-1 maintains the plasticity of basal bronchial epithelial cells.