Melan-a Cell Line
- Name Dorothy Bennett
- Institute St George's University of London
Tool name: Melan-a Cell Line
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: Mouse melanoblasts
Cancer type: Melanoma
Description: Melan-a cell line with use to investigate melanoma skin cancer.
Background and Research Application
Skin cancers are skin growths with differing causes and varying degrees of malignancy. The three most common malignant skin cancers are basal cell carcinoma, squamous cell carcinoma, and melanoma. To understand what is abnormal in melanomas, it is clearly desirable to make cellular and molecular comparisons between melanoma cells and the equivalent normal cells, melanocytes. The melanocyte line, melan-a, is derived from the embryonic skin of C57BL mice. Cells retain all tested characteristics of normal melanocytes except senescence and a proliferative response to cholera toxin in the presence of tetradecanoyl phorbol acetate (TPA). They have the diploid chromosome number and are non-tumorigenic in syngeneic and nude mice. They are therefore suitable for a range of comparative studies, in vitro and in vivo, with sublines of the B16 melanoma with which they are also syngeneic.
Research area: Cancer ; Cell Cycle ; Cell Type or Organelle Marker ; Developmental Biology ; Drug Discovery & Development
- For Research Use Only
- • Bennett et al. 1987. Int J Cancer. 39(3):414-8. PMID: 3102392.
- • A line of non-tumorigenic mouse melanocytes, syngeneic with the B16 melanoma and requiring a tumour promoter for growth.