UB-OC1 Cell Line
- Name Matthew C Holley
- Institute University of Bristol ; University Of Sheffield
- Primary citation Rivolta et al. 1998. Proc Biol Sci. 265(1406):1595-603. PMID: 9753783
Tool name: UB-OC1 Cell Line
Alternate names: Organ of Corti cell line number 1; UB/OC-1
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: Primary cultures of the developing organs of Corti of E13 embryonic Immortomouseâ?
Tissue: Developing organs of Corti
Disease: Hearing loss
Model: Conditionally immortalised
Conditional description: Proliferating cultures at 33Â°C in the presence of Î?IF and differentiating cultures at 39Â°C in the absence of Î?IF due to the temperature sensitivity of the immortalizing gene regulated by a Î?-interferoninducible promoter
Application: Inner ear development studies; Gene expression and functional studies of inner ear-specific genes; In vitro screening for gene activation and promoter analysis; Ototoxicity studies (prescribed drugs and agents that ameliorate their affects); Functional studies of inherited deafness mutations
Description: A conditionally immortal cell line derived from the mouse cochlea. The immortalizing gene was activated in organotypic cultures of auditory sensory epithelia at E13, the thirteenth day of embryonic development, before the hair cells had started to differentiate after their last mitoses.
The cell line expresses characteristic hair cell markers including the transcription factor Brn3.1, the a9 subunit of the acetylcholine receptor, the stereociliary protein fimbrin and the myosins VI and VIIA.
The relatively low expression of Brn3.1, the a9AChR and myosin VIIa in UB/OC-1 compared with UB/OC-2 at 33Â°C suggests that the former may have been immortalized at an earlier stage of differentiation.
UB/OC-1 probably originates from the nonsensory epithelial cell population in the GER and, when differentiated, closely resembles the hair cell phenotype. Thus it has considerable potential for studying the genetic processes underlying a potential mechanism for recruitment of replacement hair cells.
Research area: Developmental biology; Drug development; Genetics
Production details: Cells derived from C57/Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the Î?âinterferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the Immortomouseâ? (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100)
Cellosaurus ID: CVCL_9636
- For Research Use Only
- • Fritzsche et. al. Int J Mol Sci. 2022 May 21, 23(10):5780. PMID: 35628594
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- • Jagger et al. 2000. J Physiol. 527 Pt 1:49-54. PMID: 11011664
- • Jagger et al. 1999. Pflugers Arch. 438(1):8-14. PMID: 10370081
- • Rivolta et al. 1998. Proc Biol Sci. 265(1406):1595-603. PMID: 9753783