UB-OC2 Cell Line
- Name Matthew C Holley
- Institute University of Bristol; University of Sheffield
Tool name: UB-OC2 Cell Line
Alternate names: Organ of Corti cell line number 2
Tool type: Cell Lines
Model: Immortalised Line
Conditional description: Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the ĂĹĂ˘ÂÂinterferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the ImmortomouseĂ˘ÂË (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100)
Description: Derived from cochlear epithelium (organ of Corti), most likely the greater epithelial ridge (GER), at embryonic day E13.5 (plug in mouse designated E0.5 and birth at E18-19). At this stage the sensory hair cells have not differentiated and cells from the GER retain the capacity to differentiate as hair cells. UB/OC-2 was characterised by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. These include Cytokeratin, Vimentin, Myosin VIIa, Myosin VI, Brn3c, ĂÄ
10AChR and a combination of functional ion channels (see references). Screened with Affymetrix mouse Micro-arrays. The cells differ from UB/OC-1 by expressing more differentiation markers (eg Myosin VI1a and ĂÄ
9AChR) under immortalising conditions. Thus they are easier for functional studies on these molecules.
Epithelial cell line suitable for studies on:
Inner ear development
Gene expression and function of inner ear-specific genes
In vitro screening for gene activation and promoter analysis
Ototoxicity (prescribed drugs and agents that ameliorate their affects)
Function of inherited deafness mutations
Functional analysis of ion channels, receptors and signalling pathways in vitro
Research area: Developmental Biology ; Drug Discovery & Development ; Gene Expression
- For Research Use Only
- • Clough et al. 2004. Biochem Biophys Res Commun. 324(1):372-81. PMID: 15465029.
Jagger et al. 2000. J Physiol. 527(Pt 1):49-54. PMID: 10944169.
Jagger et al. 1999. Pflugers Arch. 438(1):8-14. PMID: 10370081.
Rivolta et al. 1998. Proc Biol Sci. 265(1406):1595-603. PMID: 9753783.