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Contributor Information

  • Name Matthew C Holley
  • Institute University of Bristol; University of Sheffield

Tool Details

  • Tool name: UB-OC2 Cell Line
  • Alternate names: Organ of Corti cell line number 2
  • Tool type: Cell Lines
  • Organism: Mouse
  • Model: Immortalised Line
  • Conditional: Yes
  • Conditional description: Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the γ–interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the Immortomouse™ (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100)
  • Description: Derived from cochlear epithelium (organ of Corti), most likely the greater epithelial ridge (GER), at embryonic day E13.5 (plug in mouse designated E0.5 and birth at E18-19). At this stage the sensory hair cells have not differentiated and cells from the GER retain the capacity to differentiate as hair cells. UB/OC-2 was characterised by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. These include Cytokeratin, Vimentin, Myosin VIIa, Myosin VI, Brn3c, Îą9AChR/Îą10AChR and a combination of functional ion channels (see references). Screened with Affymetrix mouse Micro-arrays. The cells differ from UB/OC-1 by expressing more differentiation markers (eg Myosin VI1a and Îą9AChR) under immortalising conditions. Thus they are easier for functional studies on these molecules. Epithelial cell line suitable for studies on: Inner ear development Gene expression and function of inner ear-specific genes In vitro screening for gene activation and promoter analysis Ototoxicity (prescribed drugs and agents that ameliorate their affects) Function of inherited deafness mutations Functional analysis of ion channels, receptors and signalling pathways in vitro
  • Research area: Developmental Biology ; Drug Discovery & Development ; Gene Expression

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Passage number: Passage 22
  • Growth medium: MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine, 33°C
  • Storage medium: Pure Foetal Calf serum with 10% DMSO
  • Storage conditions: Store in LN2 after a short period at -80C
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Clough et al. 2004. Biochem Biophys Res Commun. 324(1):372-81. PMID: 15465029. Jagger et al. 2000. J Physiol. 527(Pt 1):49-54. PMID: 10944169. Jagger et al. 1999. Pflugers Arch. 438(1):8-14. PMID: 10370081. Rivolta et al. 1998. Proc Biol Sci. 265(1406):1595-603. PMID: 9753783.