CELL LINES
Contributor Information
- Name Matthew C Holley
- Institute University of Bristol; University of Sheffield
- Primary citation Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448
Tool Details
- Tool name: UB-UE1 Cell Line
- Alternate names: UB-UE1; UB/UE-1; University of Bristol/Utricular Epithelium-1; Utricular Epithelium cell line number 1
- Tool type: Cell Lines
- Parental cell line: pure utricular sensory epithelia of 2-day old immortomouse pups
- Organism: Mouse
- Model: Immortalised Line
- Conditional: Yes
- Conditional description: Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the ÄÂÄšÂÄËĂÂĂÂinterferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the ImmortomouseÄËĂÂĂ (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100).
- Description: Derived from vestibular epithelium (utricular macula) using thermolysin treatment and dissection to ensure origin from sensory epithelial supporting cells at post-natal day P2. At this stage the supporting cells are normally post-mitotic but retain the ability to differentiate as sensory hair cells and or supporting cells. UB/UE-1 was characterised by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. These include Cytokeratin, Vimentin, Myosin VIIa, Myosin VI, Brn3c, ÄÂĂÂ 9AChR and a combination of functional ion channels (see references). Screened with Affymetrix mouse Micro-arrays. The cells differentiate neonatal stage hair cell and supporting cell phenotypes under differentiating conditions in vitro. Epithelial cell line suitable for studies on: Inner ear development Gene expression and function of inner ear-specific genes In vitro screening for gene activation and promoter analysis Ototoxicity (prescribed drugs and agents that ameliorate their affects) Function of inherited deafness mutations Functional analysis of ion channels, receptors and signalling pathways in vitro
- Research area: Developmental Biology; Drug Discovery & Development; Gene Expression
- Cellosaurus ID: CVCL_D807
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine, 33Ă°C
- Volume: 1 ml
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
- Available on request
Related Tools
References
- • Hackett et al. 2002. Exp Cell Res. 278(1):19-30. PMID: 12126954
- • Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448
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