CELL LINES
Contributor Information
- Name Peter Lane
- Institute University of Birmingham
- Primary citation Flynn et al. 1998. J Exp Med. 188(2):297-304. PMID: 9670042
Tool Details
- Tool name: J558L Cell Line secreting OX40L
- Alternate names: OX4L ligand, CD134, dendritic cells, DCs, Th2, CD525, systemic lupus erythematosus, TXGP1, OX-4L, gp34, TNFSF4, CD4 T cells, cytokine, Tumor necrosis factor receptor superfamily member 4, TNFRSF4
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: J558L - mammalian plasmacytoma cell line
- Organism: Mouse
- Conditional: No
- Description: OX40 receptor also known as CD134, a member of the tumor necrosis factor receptor superfamily of receptors, is not constitutively expressed on resting naive T cells. OX40 is activated 24 to 72 hours after binding to its ligand; OX40L, and is a secondary co-stimulatory immune checkpoint molecule. Expression of OX40 is dependent on full activation of the CD28 absent-T cell.
- Research area: Immunology
- Production details: Primers specific to the murine sequence of the OX40L gene were used to generate a cDNA amplicon which was cloned into an expression vector. The vector has resistant markers to ampicillin and histidinol, and also contains promoters and enhancers for murine B-cells and plasma cells. A chimeric IgG1 and OX40 molecule with a 3' splice donor site was generated by subcloning the OX40L amplicon into a human IgG1 expression vector.
- For Research Use Only
Target Details
- Target: Murine OX40L
Application Details
Handling
- Format: Frozen
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Flynn et al. 1998. J Exp Med. 188(2):297-304. PMID: 9670042
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