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Contributor Information

  • Name Nicola Curtin
  • Institute Northern Institute For Cancer Research, Newcastle University

Tool Details

  • Tool name: NUCOLL43 Cell Line
  • Alternate names: O-CCC, ovarian clear cell carcinoma, HGSC, high grade ovarian cancer, TP53, CA125, LOH, loss of heterozygosity
  • Tool type: Cell Lines
  • Tool sub-type: Primary
  • Parental cell line: Clear cell adenocarcinoma of gynecological origin
  • Organism: Human
  • Tissue: Ovary
  • Gender: Female
  • Cancer type: Gynaecologic cancer
  • Disease: Cancer
  • Growth properties: Doubling time approximately 45 hours
  • Model: Cancer Model
  • Conditional: No
  • Description: Ovarian cancer is a significant cause of death in women worldwide with the majority of ovarian cancers forming in women under the age of 65. Due to late stage diagnosis and a lack of reliable screening tests, survival rates after 5 years are below 50% in developing counties. Ovarian cancer can spread to tissues in close proximity to the ovaries, including the lining of the abdomen, lungs, lymph nodes and liver. Ovarian cancer has a number of histological subtypes and ovarian clear cell carcinomas generally respond poorly to chemotherapy. NUCOLL43 was established from ascitic fluid from a female 57 year old white British patient without artifical immortalisation. Cells display a complete loss of TP53 expression and function despite having no loss of chromosome 17p and show highly similar pan-genomic similarity to the original patient tumor. Like the original tumor, the cells also expressed epithelial and mesenchymal characteristics. NUCOLL43 cells poses a very high degree of loss of heterozygosity which affected 85% of the genome. NUCOLL43 cells are resistant to cisplatin and rucaparib, but sensitive to paclitaxel, camptothecin and NVP-BEZ 235.
  • Research area: Cancer; Cell biology; Genetics

  • For Research Use Only

Target Details

Application Details

  • Application notes: NUCOLL43 was established from ascitic fluid from a female 57 year old white British patient without artifical immortalisation. Cells display a complete loss of TP53 expression and function despite having no loss of chromosome 17p and show highly similar pan-genomic similarity to the original patient tumor. Like the original tumor, the cells also expressed epithelial and mesenchymal characteristics. NUCOLL43 cells poses a very high degree of loss of heterozygosity which affected 85% of the genome. NUCOLL43 cells are resistant to cisplatin and rucaparib, but sensitive to paclitaxel, camptothecin and NVP-BEZ 235.

Handling

  • Format: Frozen
  • Growth medium: RPMI-1640 media supplemented with 20% FBS and cultured at 37?‚°C in 5% CO2.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Franklin et al. 2018. Cancer Med. :. PMID: 30109783.
  • •  Characterization and drug sensitivity of a novel human ovarian clear cell carcinoma cell line genomically and phenotypically similar to the original tumor.