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Contributor Information

  • Name Richard Ferrero
  • Institute Hudson Institute of Medical Research

Tool Details

  • Tool name: Human AGS NOD1 KO (Clone 41A8) Cell Line
  • Alternate names: Human nucleotide-binding oligomerization domain-containing protein 1, NOD1.
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: AGS
  • Organism: Human
  • Tissue: Gastric
  • Cancer type: Gastric Adenocarcinoma
  • Disease: Cancer
  • Description: NOD1 responds to Helicobacter pylori infection in a Type IV secretion system-dependent manner. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 (as generated by CRISPR) can be used to model gastric adenocarcinoma in the context of various pathogens. NOD1 regulates host epithelial cell responses. Upregulates antimicrobial peptides of the beta-defensin (hBD) family secreted by epithelial cells.
  • Research area: Cancer ; Cell Signaling & Signal Transduction ; Immunology
  • Additional notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: RPMI with: 10% foetal calf serum; 1% penicillin-streptomycin; and 1% L-glutamine. Subculture cells every 2-3 days in ratios of 1:2-1:4 in T25 or T75 flasks.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Tran et al. 2018. Cell Microbiol. 20(5):e12826. PMID: 29392836.
  •   NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells.