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Contributor Information

  • Name Richard Ferrero
  • Institute Hudson Institute of Medical Research

Tool Details

  • Tool name: Human AGS NOD1 KD (AGS cl.1) Cell Line
  • Alternate names: Human nucleotide-binding oligomerization domain-containing protein 1, NOD1
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: AGS
  • Organism: Human
  • Tissue: Gastric
  • Cancer type: Digestive / Gastrointestinal cancer
  • Disease: Cancer
  • Description: NOD1 regulates host epithelial cell responses. Upregulates antimicrobial peptides of the beta-defensin (hBD) family secreted by epithelial cells. NOD1 is an intracellular pattern recognition receptor that mediates innate and adaptive immunity. NOD1 specifically recognises molecules with a D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety, such as the Gram-negative bacterial cell wall component, peptidoglycan, which results in the activation of the NF-kB pathway and induction of cytokines via RIPK2 and IKK-gamma. Confers reactivity to bacterial liposaccharides (LPS) and confers immunity to viral and bacterial pathogens. These cell lines have for example been used to study NOD1 responds to Helicobacter pylori infection. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 can be used to model gastric adenocarcinoma in the context of various pathogens.
  • Research area: Cancer; Cell signaling and signal transduction; Immunology
  • Production details: Human AGS NOD1 Knock-down (KD) cells were generated by integration of an expression vector containing a small interference RNA (siRNA) directed to the gene encoding the CARD domain of the NOD1 gene. Recommended Freezing Composition: 90% (v/v) FCS - 10% (v/v) DMSO

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: RPMI with: 10% foetal calf serum; 1% penicillin-streptomycin; and 1% L-glutamine. Subculture cells every 2-3 days in ratios of 1:2-1:4 in T25 or T75 flasks.
  • Storage medium: 90% (v/v) FCS - 10% (v/v) DMSO
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

References

  •   Grubman et al. 2010. Cell Microbiol. 12(5):626-39. PMID: 20039881.
  •   The innate immune molecule, NOD1, regulates direct killing of Helicobacter pylori by antimicrobial peptides.