CELL LINES
Contributor Information
- Name Aloysius Klingelhutz
- Institute The University of Iowa
Tool Details
- Tool name: 16E6-E7 HFK
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: Human foreskin keratinocytes (HFKs) established from foreskin tissue obtained from newborns
- Organism: Human
- Disease: Cancer
- Model: Immortalised Line
- Conditional: No
- Application: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
- Description: Expresses skin cell markers; proinflammatory. Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
- Research area: Cancer
- Production details: HPV-16 E7 expressing keratinocytes were generated by infecting normal primary cells with an LXSH retrovirus that expressed E7 and conferred hygromycin resistance. After selection in hygromycin, cells were infected with an LXSN retroviral construct that expressed wild-type HPV-16 E6 and conferred G418 resistance.ĂÂ
- For Research Use Only
Target Details
- Target: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
Application Details
- Application: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
Handling
- Format: Frozen
- Growth medium: Maintained in Keratinocyte-SFM (Gibco-BRL, Rockville, MD). G418 selection.
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
- Available on request
Related Tools
References
- • Duffy et al. 2003. Virology. 314(1):196-205. PMID: 14517073.
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