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Contributor Information

  • Name Aloysius Klingelhutz
  • Institute The University of Iowa

Tool Details

  • Tool name: 16E6-E7 HFK
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Human foreskin keratinocytes (HFKs) established from foreskin tissue obtained from newborns
  • Organism: Human
  • Disease: Cancer
  • Model: Immortalised Line
  • Conditional: No
  • Application: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
  • Description: Expresses skin cell markers; proinflammatory. Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
  • Research area: Cancer
  • Production details: HPV-16 E7 expressing keratinocytes were generated by infecting normal primary cells with an LXSH retrovirus that expressed E7 and conferred hygromycin resistance. After selection in hygromycin, cells were infected with an LXSN retroviral construct that expressed wild-type HPV-16 E6 and conferred G418 resistance.Â?‚ 

  • For Research Use Only

Target Details

  • Target: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies

Application Details

  • Application: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies

Handling

  • Format: Frozen
  • Growth medium: Maintained in Keratinocyte-SFM (Gibco-BRL, Rockville, MD). G418 selection.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

References

  •   Duffy et al. 2003. Virology. 314(1):196-205. PMID: 14517073.