CELL LINES
Contributor Information
- Name Michael Apicella
- Institute The University of Iowa
Tool Details
- Tool name: THCECb Cell Line
- Tool type: Cell Lines
- Organism: Human
- Tissue: Cervix
- Disease: Neisseria gonorrhoeae infection; Gonorrhea
- Model: Immortalised Line
- Conditional: No
- Description: This cell line was developed to study Neisseria gonorrhoeae (Gonorrhea) infection in women. Women with N. gonorrhoeae are often asymptomatic leading to chronic infections, upper genital tract infections leading to pelvic inflammatory disease, and increase risk for contracting HIV. This cell line has been used in studying biofilm production of N. gonorrhoeae which can exacerbate the illness and infection. This cell line expresses complement receptor type III on the surface and secretes complement components, necessary for gonococcal attachment and invasion of cervical epithelial cells.
- Research area: Immunology
- Production details: This cell line was derived from primary cervical cells obtained from cervical biopsies and immortalized with E6/E7.
- For Research Use Only
Target Details
- Target: Sexually transmitted diseases (STIs)
Application Details
Handling
- Format: Frozen
- Growth medium: Serum-free keratinocyte growth media (K-SFM) supplement 500 mL of basal media with 12.5 mg BPE and 0.08 ug EGF. Media may be supplemented with 15 PenStrep for routine maintenance.
- Shipping conditions: Dry ice
Documentation
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