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Contributor Information

  • Institute Medical Research Council

Tool Details

  • Tool name: NS0 Cell Line
  • Tool type: Cell Lines
  • Parental cell line: NS1 (P3/NS1/1-Ag4-1)
  • Organism: Mouse
  • Disease: Murine myeloma
  • Growth properties: Suspension
  • Description: A non-secreting cell line used to generate antibody secreting hybridoma cell lines. Background and Research Application NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1). The cells are resistant to 10ÂľM azaguanine, cells die in the presence of HAT medium. NS0 is widely used as a fusion partner to generate antibody secreting hybridoma cell lines and is also used as a model B-cell line. The NS0 cell line is the property of MRC. CancerTools.org is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from MRC, negotiated by LifeArc (formerly known as MRC Technology); requests for commercial use of this cell line will be forwarded to LifeArc. Points of Interest NS0 Cell Line from murine myeloma has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones.
  • Cellosaurus ID: CVCL_3940

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Culture medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes

Documentation

  • Available on request

References