CELL LINES
Contributor Information
- Name Fredrick Domann
- Institute The University Of Iowa
Tool Details
- Tool name: SOD2 Cell Line
- Alternate names: SOD2
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HEK293T
- Disease: Used as a mitochondria defective model
- Conditional: No
- Description: The SOD2-null HEK293T cell line (as generated by CRISPR) is the first known human cell line that completely lacks the enzyme responsible for removing mitochondrial superoxide generated during respiration. The resulting cells will provide an invaluable model for investigators across multiple disciplines as they search for ways to improve and boost mitochondrial function. This cell line has both basic research and translational research potential since many people have mitochondrial diseases and this new cell line will serve as an important model system to explore agents and treatments that can augment impaired mitochondrial function in multiple disciplines such as cancer, diabetes and metabolism. Used as a mitochondria defective model
- Research area: Cancer; Drug development
- Production details: Targeted gene editing by CRISPR/Cas9
- Additional notes: CRISPR edited HEK293T cells. Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.
- For Research Use Only
Target Details
- Target: Superoxide dismutase 2
Application Details
- Application notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license
Handling
- Format: Frozen
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Cramer-Morales et al. 2015. Free Radic Biol Med. 89:379-86. PMID: 26208779.
- • SOD2 targeted gene editing by CRISPR/Cas9 yields Human cells devoid of MnSOD.
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