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Contributor Information

  • Name Pranab K Das
  • Institute Amsterdam University
  • Primary citation Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834

Tool Details

  • Tool name: Immortalised Human Melanocyte [PIG1] Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Human foreskin
  • Organism: Human
  • Tissue: Neonatal foreskin
  • Gender: Male
  • Cancer type: Skin cancer
  • Disease: Pigmentary disorders
  • Model: Immortalised Line
  • Conditional: No
  • Application: Melanocyte biology research, in vitro studies on the etiology of pigmentary disorders and melanoma
  • Description: Immortalized human melanocyte cell line established by introduction of retroviral construct carrying a geneticin resistance gene and containing HPV16E6E7 open reading frames
  • Research area: Cancer; Cell biology
  • Production details: Melanocyte cultures were established from neonatal foreskin using standard methods. Melanocytes from passage 12 were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In addition the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.
  • Cellosaurus ID: CVCL_S410

  • For Research Use Only

Target Details

Application Details

  • Application: Melanocyte biology research, in vitro studies on the etiology of pigmentary disorders and melanoma

Handling

  • Format: Frozen
  • Growth medium: Ham's F10 medium supplemented with 10ng/ml tetradecanoly phorbol 13-acetate (TPA), 0.1mM 3-isobutyl-methyl-xanthine (IBMX), 1% vol/vol Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100 ug/ml streptomycin
  • Volume: 1 ml
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Characterisation tests: E6E7 expression, proliferation rate, anchorage independent growth, expression of melanoma markers, melanin synthesis, and ploidy
  • Cultured in antibiotics?: Penicillin / Streptomycin
  • Mycoplasma free: Yes
  • Biosafety level: 1
  • Subculture routine: Cells were routinely passaged 1:4 at confluency

Documentation

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References

  • •  Ivanova et al. 2008. In Vitro Cell Dev Biol Anim. 44(8-9):385-95. PMID: 18594937
  • •  Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834