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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Immortalised Human Melanocyte [PIG21] Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Human foreskin
  • Organism: Human
  • Growth properties: Cells were routinely passaged 1:4 at confluency
  • Model: Immortalised Line
  • Conditional: No
  • Description: Melanocytes are the cell of origin for a number of conditions including melanoma an aggressive type of skin cancer and vitiligo a long-term condition characterised by patches of the skin losing their pigment. Melanoma is the most dangerous type of skin cancer. Globally, in 2012, it newly occurred in 232,000 people. In 2015 there were 3.1 million with active disease which resulted in 59,800 deaths. Australia and New Zealand have the highest rates of melanoma in the world. While Vitiligo affects 1% of the worlds population with no known cause. Immortalised melanocytes can be used as a tool for research into different melanocytic lineages
  • Research area: Cancer; Cell biology
  • Production details: Melanocyte cultures were established from neonatal foreskin using standard methods. Melanocytes from passage 16 were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In addition the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Ham's F10 medium supplemented with 10ng/ml tetradecanoly phorbol 13-acetate (TPA), 0.1mM 3-isobutyl-methyl-xanthine (IBMX), 1% vol/vol Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100?„?žg/ml streptomycin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Ivanova et al. 2008. In Vitro Cell Dev Biol Anim. 44(8-9):385-95. PMID: 18594937.
  • •  Immortalization of human melanocytes does not alter the de novo properties of nitric oxide to induce cell detachment from extracellular matrix components via cGMP.