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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Immortalised CD40 deficient B Cell Line
  • Alternate names: Tumour Necrosis Factor Receptor Super family Member 5, CD4L Receptor, B Cell Surface Antigen CD4, TNFRSF5
  • Tool type: Cell Lines
  • Parental cell line: B cells from CD40 deficient patient
  • Organism: Human
  • Disease: Hyper IgM Syndrome type 3
  • Growth properties: Maintain at 3x105 to 7x105 cells per ml for optimal growth. Replenish growth medium twice per week
  • Model: Immortalised Line
  • Conditional: No
  • Description: Can be used as antigen presenting cells. Hyper IgM syndromes is a group of primary immune deficiency disorders characterised by defective CD40 signalling; via B cells affecting class switch recombination (CSR) and somatic hyper mutation. Immunoglobulin (Ig) class switch recombination deficiencies are characterised by elevated serum Immunoglobulin M (IgM) levels and a considerable deficiency in Immunoglobulins G (IgG), A (IgA) and E (IgE). As a consequence, people with HIGM have decreased concentrations of serum IgG and IgA and normal or elevated IgM, leading to increased susceptibility to infections. Hyper IgM Syndrome type 3 is characterised by mutations of the CD40 gene. In this type, B cells cannot receive the signal from T cells to switch classes
  • Research area: Cell Type or Organelle Marker; Immunology
  • Production details: B Cells were isolated from PBMCs of CD40 deficient Hyper IgM Syndrome patients using standards methods. B cells were transformed with Epstein-Barr virus.

  • For Research Use Only

Target Details

Application Details


  • Format: Frozen
  • Growth medium: RPMI 1640 medium supplemented with 10% FCS, 10mM HEPES buffer, 50U/ml penicillin and 50 Äžg/ml streptomycin
  • Shipping conditions: Dry ice


  • Available on request