CELL LINES
Contributor Information
- Name Pranab K Das
Tool Details
- Tool name: Immortalised CD40 deficient B Cell Line
- Alternate names: Tumour Necrosis Factor Receptor Super family Member 5, CD4L Receptor, B Cell Surface Antigen CD4, TNFRSF5
- Tool type: Cell Lines
- Parental cell line: B cells from CD40 deficient patient
- Organism: Human
- Disease: Hyper IgM Syndrome type 3
- Growth properties: Maintain at 3x105 to 7x105 cells per ml for optimal growth. Replenish growth medium twice per week
- Model: Immortalised Line
- Conditional: No
- Description: Can be used as antigen presenting cells. Hyper IgM syndromesÄÂĂÂ is a group ofÄÂĂÂ primary immune deficiency disordersÄÂĂÂ characterised by defectiveÄÂĂÂ CD40ÄÂĂÂ signalling;ÄÂĂÂ viaÄÂĂÂ B cellsÄÂĂÂ affectingÄÂĂÂ class switch recombinationÄÂĂÂ (CSR) andÄÂĂÂ somatic hyper mutation.ÄÂĂÂ ImmunoglobulinÄÂĂÂ (Ig) class switch recombination deficiencies are characterised by elevated serumÄÂĂÂ Immunoglobulin MÄÂĂÂ (IgM) levels and a considerable deficiency inÄÂĂÂ Immunoglobulins GÄÂĂÂ (IgG),ÄÂĂÂ AÄÂĂÂ (IgA) andÄÂĂÂ EÄÂĂÂ (IgE). As a consequence, people with HIGM have decreased concentrations of serum IgG and IgA and normal or elevated IgM, leading to increased susceptibility to infections. Hyper IgM Syndrome type 3 is characterised by mutations of the CD40 gene. In this type, B cells cannot receive the signal from T cells to switch classes
- Research area: Cell Type or Organelle Marker; Immunology
- Production details: B Cells were isolated from PBMCs of CD40 deficient Hyper IgM Syndrome patients using standards methods. B cells were transformed with Epstein-Barr virus.
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: RPMI 1640 medium supplemented with 10% FCS, 10mM HEPES buffer, 50U/ml penicillin and 50 ĂĹžg/ml streptomycin
- Shipping conditions: Dry ice
Documentation
- Available on request
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