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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Immortalised AID deficient B Cell Line
  • Alternate names: Activation-induced Cytidine Deaminase, AICDA
  • Tool type: Cell Lines
  • Parental cell line: B cells from AID deficient patient
  • Organism: Human
  • Disease: Hyper IgM Syndrome type 2
  • Growth properties: Maintain at 3x105 to 7x105 cells per ml for optimal growth. Replenish growth medium twice per week
  • Model: Immortalised Line
  • Conditional: No
  • Description: Can be used as antigen presenting cells. Hyper IgM syndromesÂ?‚ is a group ofÂ?‚ primary immune deficiency disordersÂ?‚ characterised by defectiveÂ?‚ CD40Â?‚ signalling;Â?‚ viaÂ?‚ B cellsÂ?‚ affectingÂ?‚ class switch recombinationÂ?‚ (CSR) andÂ?‚ somatic hyper mutation.Â?‚ ImmunoglobulinÂ?‚ (Ig) class switch recombination deficiencies are characterised by elevated serumÂ?‚ Immunoglobulin MÂ?‚ (IgM) levels and a considerable deficiency inÂ?‚ Immunoglobulins GÂ?‚ (IgG),Â?‚ AÂ?‚ (IgA) andÂ?‚ EÂ?‚ (IgE). As a consequence, people with HIGM have decreased concentrations of serum IgG and IgA and normal or elevated IgM, leading to increased susceptibility to infections. Hyper IgM Syndrome type 2 (autosomal recessive), is characterised by mutations of the AICDA gene. In this type, B cells cannot recombine genetic material to change heavy chain production
  • Research area: Cell biology; Immunology
  • Production details: B Cells were isolated from PBMCs of AID deficient Hyper IgM Syndrome patients using standards methods. B cells were transformed with Epstein-Barr virus.

  • For Research Use Only

Target Details

Application Details


  • Format: Frozen
  • Growth medium: RPMI 1640 medium supplemented with 10% FCS, 10mM HEPES buffer, 50U/ml penicillin and 50 ?„?žg/ml streptomycin
  • Shipping conditions: Dry ice