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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Piebaldism Melanocyte Cell Line
  • Tool type: Cell Lines
  • Parental cell line: Lesional skin of piebaldism patient
  • Organism: Human
  • Disease: Piebaldism
  • Model: Primary line
  • Conditional: No
  • Description: Piebaldism is a rare autosomal dominant disorder of melanocyte development. Common characteristics include a congenital white forelock, scattered normal pigmented and hypo pigmented macules and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung's disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool for research
  • Research area: Cell Type or Organelle Marker ; Immunology
  • Production details: Autologous skin was taken from patients with piebaldism using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10Âľg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100Âľg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: HAMs F10 supplemented with 10 Äžg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100Äžg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References