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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Vitiligo T Cell Line, Nonlesional
  • Tool type: Cell Lines
  • Parental cell line: Non-lesional skin of vitiligo patient
  • Organism: Human
  • Disease: Vitiligo
  • Model: Primary line
  • Conditional: No
  • Description: Vitiligo is a long-term skin condition characterised by patches of the skin losing their pigment. The patches of skin affected become white and usually have sharp margins. The exact cause of vitiligo is unknown however it is believed to be due to genetic susceptibility that is triggered by an environmental factor such that an autoimmune disease occurs. High frequencies of melanocyte-reactive cytotoxic T cells in the peripheral blood of vitiligo patients and the observed correlation between peri-lesional T-cell infiltration and melanocyte loss in situ suggest the important role of cellular autoimmunity in the pathogenesis of this disease. Primary T-cells isolated from both the peri-lesional and non-lesional skin biopsis can be used as a research tool.
  • Research area: Cell Type or Organelle Marker ; Immunology
  • Production details: T cell lines were generated from fresh skin biopsies. Biopsies were incubated in a 24 well plate coated with 10Âľg/well fibronectin to facilitate spontaneous migration of T cells from the biopsy into the Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100Âľg/ml streptomycin. After 5 days extravasated skin T cells were transferred to an uncoated 24 well plate and expanded by mitogenic stimulation with 0.05% PHA in the presence of irradiated allogeniec feeder cells consisting of PBMCs from two unrelated donors EBV transformed B cells and 10U/ml recombinant human IL-2 for 10 days.

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100 Äžg/ml streptomycin plus mitogenic stimulation with 0.05% PHA in the presence of irradiated allogeniec feeder cells consisting of PBMCs from two unrelated donors EBV transformed B cells and 10U/ml recombinant human IL-2 for 10 days.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Wankowicz-Kalinska et al. 2003. Lab Invest. 83(5):683-95. PMID: 12746478.