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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Human Melanocyte Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Primary
  • Parental cell line: Human foreskin
  • Organism: Human
  • Tissue: Skin
  • Model: Primary line
  • Conditional: No
  • Description: Melanocytes are the cell of origin for a number of conditions including melanoma an aggressive type of skin cancer and vitiligo a long-term condition characterised by patches of the skin losing their pigment. Melanoma is the most dangerous type of skin cancer. Globally, in 2012, it newly occurred in 232,000 people. In 2015 there were 3.1 million with active disease which resulted in 59,800 deaths. Australia and New Zealand have the highest rates of melanoma in the world. While Vitiligo affects 1% of the worlds population with no known cause. Primary melanocytes can be used as a tool for research into different melanocytic lineages
  • Research area: Cancer ; Cell Type or Organelle Marker
  • Production details: Melanocyte cultures were established from neonatal foreskin using standard methods.

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Ham's F10 medium supplemented with 10ng/ml tetradecanoly phorbol 13-acetate (TPA), 0.1mM 3-isobutyl-methyl-xanthine (IBMX), 1% vol/vol Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100g/ml streptomycin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request