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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Immortalised Human Keratinocyte Cell Line
  • Tool type: Cell Lines
  • Parental cell line: Human foreskin
  • Organism: Human
  • Tissue: Kidney
  • Growth properties: Cells were routinely passaged 1:4 at confluency
  • Model: Immortalised Line
  • Description: Immortalised human keratinocytes are widely used for studies of skin biology and differentiation
  • Research area: Cell Type or Organelle Marker
  • Production details: Keratinocyte cultures were establised from neonatal foreskin using standard methods. Keratinocytes were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Dulbecco’s Modified Eagle Medium [High Glucose, 2mM L-glutamine] supplemented with 10% Fetal Calf Serum
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References