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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Leprosy T Cell Line
  • Tool type: Cell Lines
  • Parental cell line: Skin of leprosy patient
  • Organism: Human
  • Disease: Leprosy
  • Model: Primary line
  • Description: Leprosy is an ancient disease caused by gram positive, rod shaped bacilli called Mycobacterium leprae. Patients present with varied clinico-pathological disease depending on the host immune response to Mycobacterium leprae. Lepromatous (LL) patients represent with low T cell and high humoral immune response. The Th2 paradigm is thought to underlye lepromatous disease. Primary T-cells isolated from skin biopsis can be used as a research tool.
  • Research area: Cell Type or Organelle Marker ; Immunology
  • Production details: T cell lines were generated from fresh skin biopsies. Biopsies were incubated in a 24 well plate coated with 10Âľg/well fibronectin to facilitate spontaneous migration of T cells from the biopsy into the Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100Âľg/ml streptomycin. After 5 days extravasated skin T cells were transferred to an uncoated 24 well plate and expanded by mitogenic stimulation with 0.05% PHA in the presence of irradiated allogeniec feeder cells consisting of PBMCs from two unrelated donors EBV transformed B cells and 10U/ml recombinant human IL-2 for 10 days.

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100 Äžg/ml streptomycin plus mitogenic stimulation with 0.05% PHA in the presence of irradiated allogeniec feeder cells consisting of PBMCs from two unrelated donors EBV transformed B cells and 10U/ml recombinant human IL-2 for 10 days.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References